HomeMy WebLinkAboutWood E&I MC Report 230124 (Elsinore)_Redacted
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Sample Preparation
Water Sample Freeze-Thaw
The samples were inverted for 60 seconds to mix. A subset of each sample was transferred to a
15 mL vial. Three freeze-thaw cycles were employed prior to additional sample preparation and
subsequent analysis.
Analytical Techniques
Enzyme-Linked Immunosorbent Assay (ELISA)
MCs/NODs
A microcystins/nodularins Adda ELISA (Abraxis) was utilized for the quantitative and sensitive
congener-independent detection of Adda MCs/NODs (US EPA Method 546 & Ohio EPA DES
701.0). The current method reporting limit is 15 ng/mL (ppb) based on kit sensitivity (0.75
ng/mL), minimum dilution factor (100X), and initial demonstration of capability.
Qualifier Flag
CL Analytical result is estimated due to ineffective quenching.
J Analyte was positively identified; the associated numerical value is estimated.
PT The reported result is estimated because the sample was not analyzed within required holding time.
B Analytical result is estimated. Analyte was detected in associated reagent blank as well as the samples.
E Analytical result is estimated. Values achieved were outside calibration range.
N Spiked sample control was outside limits
T The reported result is estimated because the sample exceeded temperature threshold when received
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Quality Control
Table 2: Adda MC-ELISA Quality Control Value Table
Date Analyzed: 26-Jan-23 Requirement Pass/Fail
R2 value: 0.999 ≥0.98 PASS
%CV STDs: 0.1-4.1% ≤15% PASS
LFB (1 ppb) recovery: 105% ±40% True Value PASS
%CV LFB: 3.8% ≤20% PASS
Low CCC (0.15 ppb) recovery: 117% ±50% True Value PASS
LRB <0.08 <0.08 PASS
Results
Table 3: Raw ELISA Data including the sample identification, analyte, date analyzed,
interpolated values 1 and 2, the dilution factor, %CV of the absorbance values, and average final
concentration (ng/mL; ppb).
Sample Date Value 1 Value 2 Dilution Average
Sample ID Type Analyte analyzed (ng/mL) (ng/mL) Factor %CV (ng/mL)
Elm Grove Beach FS MCs/NODs 1/26/2023 0.80 0.84 50 1.7 41.0
Launch Pointe Boat Ramp FS MCs/NODs 1/26/2023 0.22 0.22 50 0.6 11.0
Elsinore West Marina FS MCs/NODs 1/26/2023 0.24 0.24 50 0.1 12.0
Mid Lake FS MCs/NODs 1/26/2023 0.16 0.17 50 0.7 8.3