HomeMy WebLinkAboutWood E&I MC Report 221215 (Elsinore)_Redacted
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Sample Preparation
Water Sample Freeze-Thaw
The samples were inverted for 60 seconds to mix. A subset of each sample was transferred to a
15 mL vial. Three freeze-thaw cycles were employed prior to additional sample preparation and
subsequent analysis.
Analytical Techniques
Enzyme-Linked Immunosorbent Assay (ELISA)
MCs/NODs
A microcystins/nodularins Adda ELISA (Abraxis) was utilized for the quantitative and sensitive
congener-independent detection of Adda MCs/NODs (US EPA Method 546 & Ohio EPA DES
701.0). The current method reporting limit is 15 ng/mL (ppb) based on kit sensitivity (0.75
ng/mL), minimum dilution factor (100X), and initial demonstration of capability.
Qualifier Flag
CL Analytical result is estimated due to ineffective quenching.
J Analyte was positively identified; the associated numerical value is estimated.
PT The reported result is estimated because the sample was not analyzed within required holding time.
B Analytical result is estimated. Analyte was detected in associated reagent blank as well as the samples.
E Analytical result is estimated. Values achieved were outside calibration range.
N Spiked sample control was outside limits
T The reported result is estimated because the sample exceeded temperature threshold when received
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Quality Control
Table 2: Adda MC-ELISA Quality Control Value Table
Date Analyzed: 21-Dec-22 Requirement Pass/Fail
R2 value: 0.999 ≥0.98 PASS
%CV STDs: 0.3 - 8.3% ≤15% PASS
LFB (1 ppb) recovery: 99% ±40% True Value PASS
%CV LFB: 4.1% ≤20% PASS
Low CCC (0.15 ppb) recovery: 103% ±50% True Value PASS
LRB <0.08 <0.08 PASS
Results
Table 3: Raw ELISA Data including the sample identification, analyte, date analyzed,
interpolated values 1 and 2, the dilution factor, %CV of the absorbance values, and average final
concentration (ng/mL; ppb).
Sample Date Value 1 Value 2 Dilution Average
Sample ID Type Analyte analyzed (ng/mL) (ng/mL) Factor %CV (ng/mL)
Launch Pointe Boat Ramp FS MCs/NODs 12/21/2022 0.61 0.59 100 1.1 60
Elsinore West Marina FS MCs/NODs 12/21/2022 0.88 1.26 100 11.6 107
Elm Grove Beach FS MCs/NODs 12/21/2022 0.48 0.46 100 0.9 47
Launch Pointe Scum FS MCs/NODs 12/21/2022 0.25 0.23 1000 1.1 240
Elsinore West Marina Scum FS MCs/NODs 12/21/2022 0.48 0.65 1000 7.9 570
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Summary of Results
Table 4: Summary of results in ng/mL
MCs/NODs
Sample ID (ng/mL)
Launch Pointe Boat Ramp 60
Elsinore West Marina 107
Elm Grove Beach 47
Launch Pointe Scum 240
Elsinore West Marina Scum 570
MRL (ng/mL): 15
Analyst Initials: MA
Date Analyzed: 12/21/2022
Interpretations:
The levels of Adda MCs/NODs detected in the samples are significantly above the current ‘Draft
EPA Recommended Value for Recreational Criteria and Swimming Advisory,’ at 8.0 ng/mL
(ppb) total microcystins. The WHO recreational guidance value for microcystin is currently 24
ng/mL (ppb) (World Health Organization (WHO), 2020a).
Submitted by:
Mark T. Aubel, Ph.D.
Lab Director
Date: December 22, 2022
The results in this report relate only to the samples listed above.
This report shall not be reproduced except in full without written approval of the laboratory.